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cloning cep83 egfp cdna  (Addgene inc)


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    Structured Review

    Addgene inc cloning cep83 egfp cdna
    Cloning Cep83 Egfp Cdna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfpc1+cep83/pEGFPC1-Cep83+(Plasmid+%23128874)/pm41315244-427-6-11
    Average 93 stars, based on 3 article reviews
    cloning cep83 egfp cdna - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: PCM1 coordinates centrosome asymmetry with polarized endosome dynamics to regulate daughter cell fate.
    Article Snippet: .. The pcs2+CEP83-EGFP plasmid was made by cloning CEP83-EGFP cDNA from pEGFPC1-CEP83 (Addgene, #128874) into the pcs2+ plasmid vector using PCR primers: pcs2+-XbaI:TAT CTA GAT CAC TCC CCA GGA GAC CCA AGC TCC and pcs2+-BamHI: TAG GAT CCG CCA CCA TGG TGA GCA AGG GCG AGG A. Plasmids (pCS2-H2B-mRFP; pCS2-par-3-GFP; pCS2-GFP-centrin; pCS2-pcm1-linker-GFP) were linearized by the restriction enzyme NotI digestion. .. NotI-linearized plasmids were purified (QIAquick Gel Extraction Kit), and the 5’-capped mRNAs were synthesized using SP6 mMessengermMachine kit (Ambion).

    Article Title: PCM1 coordinates centrosome asymmetry with polarized endosome dynamics to regulate daughter cell fate
    Article Snippet: .. The pcs2 + -CEP83-EGFP plasmid was made by cloning CEP83-EGFP cDNA from pEGFPC1-CEP83 (Addgene, #128874) into the pcs2 + plasmid vector using PCR primers: pcs2 + -XbaI:TAT CTA GAT CAC TCC CCA GGA GAC CCA AGC TCC and pcs2 + -BamHI: TAG GAT CCG CCA CCA TGG TGA GCA AGG GCG AGG A. Plasmids (pCS2-H2B-mRFP; pCS2-par-3-GFP; pCS2-GFP-centrin; pCS2-pcm1-linker-GFP) were linearized by the restriction enzyme NotI digestion. .. NotI-linearized plasmids were purified (QIAquick Gel Extraction Kit), and the 5’-capped mRNAs were synthesized using SP6 mMessenger mMachine kit (Ambion).

    Cloning:

    Article Title: PCM1 coordinates centrosome asymmetry with polarized endosome dynamics to regulate daughter cell fate.
    Article Snippet: .. The pcs2+CEP83-EGFP plasmid was made by cloning CEP83-EGFP cDNA from pEGFPC1-CEP83 (Addgene, #128874) into the pcs2+ plasmid vector using PCR primers: pcs2+-XbaI:TAT CTA GAT CAC TCC CCA GGA GAC CCA AGC TCC and pcs2+-BamHI: TAG GAT CCG CCA CCA TGG TGA GCA AGG GCG AGG A. Plasmids (pCS2-H2B-mRFP; pCS2-par-3-GFP; pCS2-GFP-centrin; pCS2-pcm1-linker-GFP) were linearized by the restriction enzyme NotI digestion. .. NotI-linearized plasmids were purified (QIAquick Gel Extraction Kit), and the 5’-capped mRNAs were synthesized using SP6 mMessengermMachine kit (Ambion).

    Article Title: PCM1 coordinates centrosome asymmetry with polarized endosome dynamics to regulate daughter cell fate
    Article Snippet: .. The pcs2 + -CEP83-EGFP plasmid was made by cloning CEP83-EGFP cDNA from pEGFPC1-CEP83 (Addgene, #128874) into the pcs2 + plasmid vector using PCR primers: pcs2 + -XbaI:TAT CTA GAT CAC TCC CCA GGA GAC CCA AGC TCC and pcs2 + -BamHI: TAG GAT CCG CCA CCA TGG TGA GCA AGG GCG AGG A. Plasmids (pCS2-H2B-mRFP; pCS2-par-3-GFP; pCS2-GFP-centrin; pCS2-pcm1-linker-GFP) were linearized by the restriction enzyme NotI digestion. .. NotI-linearized plasmids were purified (QIAquick Gel Extraction Kit), and the 5’-capped mRNAs were synthesized using SP6 mMessenger mMachine kit (Ambion).

    Polymerase Chain Reaction:

    Article Title: PCM1 coordinates centrosome asymmetry with polarized endosome dynamics to regulate daughter cell fate.
    Article Snippet: .. The pcs2+CEP83-EGFP plasmid was made by cloning CEP83-EGFP cDNA from pEGFPC1-CEP83 (Addgene, #128874) into the pcs2+ plasmid vector using PCR primers: pcs2+-XbaI:TAT CTA GAT CAC TCC CCA GGA GAC CCA AGC TCC and pcs2+-BamHI: TAG GAT CCG CCA CCA TGG TGA GCA AGG GCG AGG A. Plasmids (pCS2-H2B-mRFP; pCS2-par-3-GFP; pCS2-GFP-centrin; pCS2-pcm1-linker-GFP) were linearized by the restriction enzyme NotI digestion. .. NotI-linearized plasmids were purified (QIAquick Gel Extraction Kit), and the 5’-capped mRNAs were synthesized using SP6 mMessengermMachine kit (Ambion).

    Article Title: PCM1 coordinates centrosome asymmetry with polarized endosome dynamics to regulate daughter cell fate
    Article Snippet: .. The pcs2 + -CEP83-EGFP plasmid was made by cloning CEP83-EGFP cDNA from pEGFPC1-CEP83 (Addgene, #128874) into the pcs2 + plasmid vector using PCR primers: pcs2 + -XbaI:TAT CTA GAT CAC TCC CCA GGA GAC CCA AGC TCC and pcs2 + -BamHI: TAG GAT CCG CCA CCA TGG TGA GCA AGG GCG AGG A. Plasmids (pCS2-H2B-mRFP; pCS2-par-3-GFP; pCS2-GFP-centrin; pCS2-pcm1-linker-GFP) were linearized by the restriction enzyme NotI digestion. .. NotI-linearized plasmids were purified (QIAquick Gel Extraction Kit), and the 5’-capped mRNAs were synthesized using SP6 mMessenger mMachine kit (Ambion).



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